Journal: Investigative Ophthalmology & Visual Science

Article Title: Porcine Sub-Retinal Pigment Epithelium Deposits: A Model for Dry Age-Related Macular Degeneration With Comparison to Human Drusen

doi: 10.1167/iovs.66.3.18

Figure Lengend Snippet: Antibody, Lectin, and Dye Concentrations, Reactivities, and Sources

Article Snippet: Collagen type VI polyclonal antibody , Collagen VI , 3D–3F , 1:100 , H, P , Thermo Fisher Scientific , Proteintech 14853-1-AP.

Techniques: Concentration Assay, Plasmid Preparation

Journal: Disease Models & Mechanisms

Article Title: Col6a1 knock-in mice provide a promising pre-clinical model for collagen VI-related dystrophies

doi: 10.1242/dmm.052460

Figure Lengend Snippet: Quantitative analysis of immunoblotting for collagen alpha 1 (VI). Bars graphs showing the relative abundance of collagen alpha 1 (VI) in triceps, diaphragm, gastrocnemius and tibialis anterior muscles of wild-type, heterozygous and homozygous mice at different ages ( n =3-6 mice per genotype per stage). Col6a1 was detected with a polyclonal antibody against collagen type VI (70R-CR009x). Data were analyzed using one-way ANOVA followed by Tukey's multiple comparison test. Data are the mean±s.e.m. * P <0.05, ** P <0.011, *** P <0.001, **** P <0.0001. WT, wild-type (Col6a1 +/+ ); HET, heterozygous (Col6a1 G292R/+ ); HOM, homozygous (Col6a1 G292R/ G292R ).

Article Snippet: Blotted membranes were blocked for 1 h in 5% milk or BSA in Tris-buffered saline [10 mM Tris, 150 mM NaCl (pH 8.0)] plus 0.1% Tween and incubated overnight at 4°C with rabbit polyclonal anti-collagen VI (Fitzgerald, #70R-CR009X) or rabbit polyclonal anti-desmin (Abcam, #ab8592) antibodies.

Techniques: Western Blot, Muscles, Comparison